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| Catalog Number | orb1806532 |
|---|---|
| Category | Assays and Kits |
| Description | General 25-Hydroxyvitamin D3 (HVD3) ELISA Kit |
| Target | HVD3 |
| Reactivity | All |
| Application notes | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 25-Hydroxyvitamin D3(HVD3) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 25-Hydroxyvitamin D3(HVD3). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 25-Hydroxyvitamin D3(HVD3) in the samples is then determined by comparing the OD of the samples to the standard curve. |
| Assay Type | Competitive |
| Assay Time | 1.5H |
| Standard | 200 ng/mL |
| Range | 3.13-200 ng/mL |
| Sensitivity | 0.91 ng/mL |
| Sample Types | serum, plasma and other biological fluids |
| Alternative names | 25-HVD3; 25HVD3; Calcifediol; Calcidiol; H-VD3; 25 Read more... |
| Note | For research use only |
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