General 1, 25-Dihydroxyvitamin D3 (DHVD3) ELISA Kit

• Catalog Number: orb1806547
• Category: Assays and Kits
• Description: General 1, 25-Dihydroxyvitamin D3 (DHVD3) ELISA Kit
• Target: DHVD3
• Reactivity: All
• Application notes: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 1,25-Dihydroxyvitamin D3(DHVD3) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 1,25-Dihydroxyvitamin D3(DHVD3). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 1,25-Dihydroxyvitamin D3(DHVD3) in the samples is then determined by comparing the OD of the samples to the standard curve.
• Assay Type: Competitive
• Assay Time: 1.5H
• Standard: 10000 pg/mL
• Range: 156.25-10000 pg/mL
• Sensitivity: 49.5 pg/mL
• Sample Types: serum, plasma and other biological fluids
• Alternative names: DH-VD3; Calcitriol; Rocaltrol; Calcijex; 1,25-(OH)
• Note: For research use only