GCH1 Antibody

• Catalog Number: orb1246545
• Category: Antibodies
• Description: GCH1 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Mouse, Rat
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-GKVHIGYLPNKQ
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx. 37kDa band observed in Human Tonsil lysates. Customer’s experiments gave identical results in lysates of cell line HUVEC with antibodies raised against different parts of the same protein. (calculated MW of 27.9kDa according to NP_000152.1). Recommended concentration: 0.5-1.5ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Brain (Cerebellum) and Small Intestine. Recommended concentration: 5ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: GCH1
• UniProt ID: P30793
• NCBI: NP_001019242.1, NP_001019195.1, NP_000152.1, NP_001019241.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: GCH1 , GTP cyclohydrolase 1 (dopa-responsive dysto
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx. 37kDa band observed in Human Tonsil lysates. Customer’s experiments gave identical results in lysates of cell line HUVEC with antibodies raised against different parts of the same protein. (calculated MW of 27.9kDa according to NP_000152.1). Recommended concentration: 0.5-1.5ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Brain (Cerebellum) and Small Intestine. Recommended concentration: 5ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1246545 (0.5 ug/ml) staining of Human Tonsil lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1246545 (5 ug/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1246545 (5 ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.