GCH1 Antibody

SKU: orb19890

Description

Goat polyclonal antibody to GCH1

Images & Validation

• Tested Applications: ELISA, IHC
• Dilution range: ELISA: 1:16000, WB: 0.5-1.5 μg/ml, IHC-P: 5 μg/ml
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx. 37kDa band observed in Human Lymph Node, Thymus and Tonsil lysates. Customer's experiments gave identical results in lysates of cell line HUVEC with antibodies raised against different parts of the same protein. (calculated MW of 27.9kDa according to NP_000152.1). Recommended concentration: 1-3 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: GCH1
• Molecular Weight: 27.9; 25.8; 23.5
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti GCH1 antibody, anti GTP cyclohydrolase 1 (dopa-responsive dystonia) antibody, anti DYT5 antibody, anti GCH antibody, anti GTP-CH-1 antibody, anti GTPCH1 antibody, anti GTP cyclohydrolase 1 antibody, anti guanosine 5\1-triphosphate cyclohydrolase I antibody

orb19890 (1ug/ml) staining of Human Thymus lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemical staining of Human Small Intestine using GCH1 antibody

Immunohistochemical staining of Human Cerebellum using GCH1 antibody

Quick Database Links

Gene Symbol

GCH1

NCBI Gene ID

2643