GATA1 Antibody

• Catalog Number: orb1247458
• Category: Antibodies
• Description: GATA1 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IF, WB
• Predicted Reactivity: Bovine, Canine, Rat
• Reactivity: Human, Mouse
• Immunogen: The immunogen for this antibody is: C-DAEAYRHSPVFQ
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 50-55kDa band observed in nuclear lysates of cell line K562 calculated MW of 42.8kDa according to NP_002040.1). The observed molecular weight corresponds to different antibodies from other commercial sources. Recommended concentration: 0.3-1ug/ml. Primary incubation 1 hour at room temperature. Negative Control: Human Hippocampus lysate.Immunofluorescence: Strong expression of the protein seen in the nuclei of HeLa and NIH3T3 cells. Recommended concentration: 10ug/ml. Flow Cytometry: Flow cytometric analysis of K562 cells. Recommended concentration: 10ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: GATA1
• UniProt ID: P15976
• NCBI: NP_002040.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: GATA1, GATA binding protein 1 (globin transcriptio
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 50-55kDa band observed in nuclear lysates of cell line K562 calculated MW of 42.8kDa according to NP_002040.1). The observed molecular weight corresponds to different antibodies from other commercial sources. Recommended concentration: 0.3-1ug/ml. Primary incubation 1 hour at room temperature. Negative Control: Human Hippocampus lysate.Immunofluorescence: Strong expression of the protein seen in the nuclei of HeLa and NIH3T3 cells. Recommended concentration: 10ug/ml. Flow Cytometry: Flow cytometric analysis of K562 cells. Recommended concentration: 10ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1247458 (1 ug/ml) staining of K562 nuclear cell lysate (A) and negative control Human Hippocampus (B) lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1247458 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing nuclear staining.

orb1247458 Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing nuclear staining. The nuclear stain is DAPI.

orb1247458 Flow cytometric analysis of paraformaldehyde fixed K562 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line).