GATA1 Antibody

SKU: orb1247458

Description

GATA1 Antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: ELISA, FC, IF, WB
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Canine, Rat
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 50-55kDa band observed in nuclear lysates of cell line K562 calculated MW of 42.8kDa according to NP_002040.1). The observed molecular weight corresponds to different antibodies from other commercial sources. Recommended concentration: 0.3-1µg/mL. Primary incubation 1 hour at room temperature. Negative Control: Human Hippocampus lysate.Immunofluorescence: Strong expression of the protein seen in the nuclei of HeLa and NIH3T3 cells. Recommended concentration: 10µg/mL. Flow Cytometry: Flow cytometric analysis of K562 cells. Recommended concentration: 10µg/mL.

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Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-DAEAYRHSPVFQ
• Target: GATA1
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

GATA1, GATA binding protein 1 (globin transcription factor 1), HGNC:4170, ERYF1, GF1, NFE1, GATA binding protein 1, GATA-binding protein 1 (globin transcription factor 1), erythrold transcription factor 1, globin transcription factor 1, transcription factor GATA1, GF-1, XLTT, NF-E1 DNA-binding protein, erythroid transcription factor 1

Quick Database Links

Gene Symbol

GATA1

UniProt

P15976

RefSeq (Protein):NP_002040.1