GARS Antibody

• Catalog Number: orb1257494
• Category: Antibodies
• Description: GARS Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 60-370 of human GARS (NP_002038.2).
• Concentration: batch dependent
• Dilution range: WB: 1:1000 - 1:4000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 83kDa
• Target: GARS
• UniProt ID: P41250
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: CMT2D, DSMAV, GlyRS, HMN5, SMAD1, glycine--tRNA li
• Note: For research use only
• Application notes: WB: 1:1000 - 1:4000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Immunohistochemistry of paraffin-embedded rat brain using GARS antibody (orb1257494) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using GARS antibody (orb1257494) at 1:5000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.

Immunohistochemistry of paraffin-embedded mouse brain using GARS antibody (orb1257494) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of NIH/3T3 cells using GARS antibody (orb1257494) at dilution of 1:100. Blue: DAPI for nuclear staining.