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Catalog Number | orb1257494 |
---|---|
Category | Antibodies |
Description | GARS Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 60-370 of human GARS (NP_002038.2). |
Antibody Type | Primary Antibody |
Concentration | batch dependent |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 83kDa |
Target | GARS |
UniProt ID | P41250 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | Liquid |
Alternative names | CMT2D, DSMAV, GlyRS, HMN5, SMAD1, glycine--tRNA li Read more... |
Note | For research use only |
Application notes | WB: 1:1000 - 1:4000IHC: 1:50 - 1:200IF: 1:50 - 1:200 |
Expiration Date | 12 months from date of receipt. |
Immunohistochemistry of paraffin-embedded rat brain using GARS antibody (orb1257494) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using GARS antibody (orb1257494) at 1:5000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.
Immunohistochemistry of paraffin-embedded mouse brain using GARS antibody (orb1257494) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of NIH/3T3 cells using GARS antibody (orb1257494) at dilution of 1:100. Blue: DAPI for nuclear staining.
ICC, IF, IHC-Fr, IHC-P, WB | |
Rat | |
Human, Mouse | |
Rabbit | |
Recombinant | |
Unconjugated |
ELISA, IF, IHC, IP, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF, IH, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |