Cart summary

You have no items in your shopping cart.

GART Recombinant Rabbit Monoclonal Antibody

Catalog Number: orb1291518

DispatchUsually dispatched within 5-10 working days
$ 270.00
Catalog Numberorb1291518
CategoryAntibodies
DescriptionGART Recombinant Rabbit Monoclonal Antibody
Species/HostRabbit
ClonalityRecombinant
Clone Number6A8
Tested applicationsICC, IF, IHC-Fr, IHC-P, WB
Predicted ReactivityRat
ReactivityHuman, Mouse
IsotypeIgG
ImmunogenRecombinant Human GART protein (570-750/1010aa)
Antibody TypeRecombinant Antibody
Concentration1mg/ml
Dilution rangeWB=1:500, IHC-P=1:100-500, IHC-F=1:400-800, ICC/IF=1:50-200, IF=1:100-500
Form/AppearanceLiquid
ConjugationUnconjugated
MW107 kDa
TargetGART
UniProt IDP22102
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Alternative names5'-phosphoribosylglycinamide transformylase; AIR s
Read more...
NoteFor research use only
Expiration Date12 months from date of receipt.
GART Recombinant Rabbit Monoclonal Antibody

ICC staining of GART in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1000 dilution. The nuclear counter stain is DAPI (blue).

GART Recombinant Rabbit Monoclonal Antibody

ICC staining of GART in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1000 dilution. The nuclear counter stain is DAPI (blue).

GART Recombinant Rabbit Monoclonal Antibody

ICC staining of GART in SW480 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (orb1291518, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1000 dilution. The nuclear counter stain is DAPI (blue).

GART Recombinant Rabbit Monoclonal Antibody

Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-GART antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH9.0) for 20 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (orb1291518, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

GART Recombinant Rabbit Monoclonal Antibody

Immunohistochemical analysis of paraffin-embedded mouse uterus tissue using anti-GART antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH9.0) for 20 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

GART Recombinant Rabbit Monoclonal Antibody

Western blot analysis of GART on different lysates with Rabbit anti-GART antibody at 1/500 dilution. Lane 1: Hela cell lysate, Lane 2: A431 cell lysate, Lysates/proteins at 10 µg/Lane. Predicted band size: 108 kDa, Observed band size: 125 kDa, Exposure time: 30 seconds, 10% SDS-PAGE gel.