GAD67 antibody

• Catalog Number: orb555997
• Category: Antibodies
• Description: Rabbit polyclonal antibody to GAD67
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat, Zebrafish
• Isotype: IgG
• Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human GAD67
• Antibody Type: Primary Antibody
• Concentration: Batch specific - Reach out to find out the current concentration
• Dilution range: WB: 1:500-1:3000 , ICC/IF: 1:100-1:1000 , IHC-P: 1:100-1:1000 , IHC-Fr: 1:100-1:1000
• Form/Appearance: PBS, 1% rAlbumin, 20% Glycerol, 0.025% ProClin 300
• Purity: Purified by antigen-affinity chromatography
• Conjugation: Unconjugated
• MW: 67 kDa
• Target: This antibody is specific for GAD67 protein, and it does not cross-react with GAD65 protein
• UniProt ID: Q99259
• Storage: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles
• Alternative names: glutamate decarboxylase 1 , CPSQ1 , GAD , SCP
• Background: This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes.
• Note: For research use only
• Application notes: Positive control: human GAD1-transfected 293T
• Expiration Date: 12 months from date of receipt.

IHC-P Image - GAD67 antibody detects GAD67 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. GAD67 stained by GAD67 antibody (orb555997) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GAD67 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody

GAD67 antibody detects GAD67 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. GAD67 stained by GAD67 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GAD67 antibody detects GAD67 protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GAD67 stained by GAD67 antibody diluted at 1:500. Blue: Fluoroshield with DAPI

GAD67 antibody detects GAD67 protein by immunofluorescent analysis. Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GAD67 stained by GAD67 antibody diluted at 1:500. Red: Tau, stained by Tau antibody diluted at 1:500. Blue: Fluoroshield with DAPI

GAD67 antibody detects GAD67 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse eye. Green: GAD67 stained by GAD67 antibody diluted at 1:250. Red: beta Tubulin 3/ Tuj1, a neural marker, stained by beta Tubulin 3/ Tuj1 antibody diluted at 1:500. Blue: Fluoroshield with DAPI Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GAD67 antibody detects GAD67 protein by western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg whole cell lysate/extract of human GAD1-transfected 293T cells 7.5% SDS-PAGE GAD67 antibody dilution: 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody

Various tissue extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GAD67 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody

GAD67 antibody detects GAD67 protein on myofiber membranes by immunohistochemical analysis. Sample: Whole-mount zebrafish embryo GAD67 antibody dilution: 1:200

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GAD67 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody

Human tissue extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with GAD67 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody

GAD67 antibody detects GAD67 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse cerebellum. GAD67 stained by GAD67 antibody diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GAD67 antibody detects GAD67 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat cerebellum. GAD67 stained by GAD67 antibody diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min