GAD67 Rabbit Polyclonal Antibody

SKU: orb555997

Description

Rabbit polyclonal antibody to GAD67

Research Area

Neuroscience

Images & Validation

• Tested Applications: ICC, IF, IHC, WB
• Dilution Range: WB: 1:500-1:3000 , ICC/IF: 1:100-1:1000 , IHC-P: 1:100-1:1000 , IHC-Fr: 1:100-1:1000
• Reactivity: Human, Monkey, Mouse, Rat, Zebrafish
• Application Notes:
  Positive control: human GAD1-transfected 293T

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human GAD67
• Target: This antibody is specific for GAD67 protein, and it does not cross-react with GAD65 protein
• Molecular Weight: 67 kDa
• Purity: Purified by antigen-affinity chromatography
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles
• Form/Appearance: PBS, 1% rAlbumin, 20% Glycerol, 0.025% ProClin 300
• Concentration: Batch specific - Reach out to find out the current concentration
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

glutamate decarboxylase 1 , CPSQ1 , GAD , SCP

IHC-P Image - GAD67 antibody detects GAD67 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. GAD67 stained by GAD67 antibody (orb555997) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GAD67 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody

GAD67 antibody detects GAD67 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. GAD67 stained by GAD67 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GAD67 antibody detects GAD67 protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GAD67 stained by GAD67 antibody diluted at 1:500. Blue: Fluoroshield with DAPI

GAD67 antibody detects GAD67 protein by immunofluorescent analysis. Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GAD67 stained by GAD67 antibody diluted at 1:500. Red: Tau, stained by Tau antibody diluted at 1:500. Blue: Fluoroshield with DAPI

GAD67 antibody detects GAD67 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse eye. Green: GAD67 stained by GAD67 antibody diluted at 1:250. Red: beta Tubulin 3/ Tuj1, a neural marker, stained by beta Tubulin 3/ Tuj1 antibody diluted at 1:500. Blue: Fluoroshield with DAPI Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GAD67 antibody detects GAD67 protein by western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg whole cell lysate/extract of human GAD1-transfected 293T cells 7.5% SDS-PAGE GAD67 antibody dilution: 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody

Various tissue extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GAD67 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody

GAD67 antibody detects GAD67 protein on myofiber membranes by immunohistochemical analysis. Sample: Whole-mount zebrafish embryo GAD67 antibody dilution: 1:200

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GAD67 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody

Human tissue extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with GAD67 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody

GAD67 antibody detects GAD67 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse cerebellum. GAD67 stained by GAD67 antibody diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GAD67 antibody detects GAD67 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat cerebellum. GAD67 stained by GAD67 antibody diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Quick Database Links

Gene Symbol

This antibody is specific for GAD67 protein, and it does not cross-react with GAD65 protein

UniProt

Q99259