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Description
Research Area
Neuroscience
Images & Validation
−Item 1 of 3
| Tested Applications | ELISA, WB |
|---|---|
| Reactivity | Human |
| Predicted Reactivity | Canine, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Goat |
| Clonality | Polyclonal |
| Immunogen | The immunogen for this antibody is: C-TLEDNEERMSRLSK |
| Target | GAD2 |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Concentration | 500 ug/mL |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−GAD2, GAD65, glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa), Glutamate decarboxylase-2 (pancreas), glutamate decarboxylase 2 (pancreatic islets and brain, 65kD), RP11-420F12.2, MGC161605, MGC161607, Glutamate decarboxylase-2 (pancreas), glutamate decarboxylase 2
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orb1247466 (0.5 ug/ml) staining of Human Brain (Hippocampus) lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.
orb1247466 (2.5 ug/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.
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Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_000809.1, NP_001127838.1 |
|---|
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Protocol Information
WB
Western Blot (IB, immunoblot)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
GAD2 Antibody (orb1247466)
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