GABA A Receptor Antibody

SKU: orb67433

Description

Mouse Anti-Rat GABAA Receptor Delta Monoclonal IgG2a

Research Area

Neuroscience

Images & Validation

• Tested Applications: AM, ICC, IF, WB
• Dilution Range: WB (1:1000), IHC-P (1:1000), ICC/IF (1:100)
• Reactivity: Human, Mouse, Rat
• Application Notes:
  2 µg/ml of SMC-345 was sufficient for detection of Delta1 GABA-A receptor in 10 µg of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone No.: N151/3 (Formerly sold as S151-3)
• Immunogen: Synthetic peptide amino acids 15-34 (N-terminus) of rat GABA-A-R-Delta
• Target: GABA A Receptor
• Molecular Weight: 55kDa
• Purification: Protein G Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS pH 7.4, 50% glycerol, 0.09% sodium azide Storage buffer changes when conjugated
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

GABAA-RD, GABRD, MGC105467, Gamma-aminobutyric acid receptor subunit delta, GABA(A) receptor subunit delta, GBRD_HUMAN

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GABA-A Receptor Delta Monoclonal Antibody, Clone N151/3. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-GABA-A Receptor Delta Monoclonal Antibody at 1:100 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) GABA-A Receptor Delta Antibody (D) Composite.

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GABA A Receptor Monoclonal Antibody, Clone N151/3. Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20°C. Primary Antibody: Mouse Anti-GABA A Receptor Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Diffuse cytoplasm and dull nuclei.

Western Blot analysis of Rat Cell line lysates showing detection of GABA A Receptor protein using Mouse Anti-GABA A Receptor Monoclonal Antibody, Clone N151/3. Load: 15 μg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-GABA A Receptor Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

Quick Database Links

Gene Symbol

GABA A Receptor

UniProt

P18506

NCBI Gene ID

29689

RefSeq (Protein):NP_058985.1