GABRA1/GABA A Receptor alpha 1 Rabbit Polyclonal Antibody

SKU: orb10677

Description

GABRA1/GABA A Receptor alpha 1 Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human GABRA1 (301-450/456aa)
• Target: GABRA1
• Molecular Weight: 52 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

ECA4; EJM; EJM5; Gaba receptor alpha 1 polypeptide; GABA(A) receptor; GABA(A) receptor subunit alpha 1; GABA(A) receptor subunit alpha-1; GABA(A) receptor, alpha 1; GABRA 1; GABRA1; gamma aminobutyric acid(GABA) A receptor alpha 1; gamma aminobutyric acid A receptor alpha 1; gamma aminobutyric acid receptor subunit alpha 1; gamma-aminobutyric acid receptor subunit alpha-1; GBRA1_HUMAN.

Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (GABRA1 GABA A Receptor alpha 1) Polyclonal Antibody, Unconjugated (orb10677) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (GABRA1 GABA A Receptor alpha 1) Polyclonal Antibody, Unconjugated (orb10677) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Brain (Mouse) Lysate at 40 ug, Primary: Anti-GABA A Receptor (orb10677) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/10000 dilution, Predicted band size: 52 kD, Observed band size: 52 kD.

Sample: Cerebrum (Mouse) Lysate at 40 ug, Cerebellum (Rat) Lysate at 40 ug, Primary: Anti-GABRA1/GABA A Receptor alpha 1 (orb10677) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 52 kD, Observed band size: 52 kD.

Quick Database Links

Gene Symbol

GABRA1

UniProt

P14867