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GABA A Receptor Alpha 5 Antibody: APC
Catalog Number: orb376825
| Catalog Number | orb376825 |
|---|---|
| Category | Antibodies |
| Description | Mouse monoclonal antibody against GABA A Receptor conjugated to APC . |
| Species/Host | Mouse |
| Clonality | Monoclonal |
| Clone Number | N415/24 (Formerly sold as S415-24) |
| Tested applications | ICC, IF, IHC |
| Reactivity | Human, Mouse, Rat |
| Isotype | IgG1 |
| Immunogen | Fusion protein amino acids 368-419 (last cytoplasmic domain before extracellular C-terminus) of human GABA A Receptor Alpha5. |
| Concentration | 1 mg/ml |
| Dilution range | WB (1:1000) |
| Conjugation | APC |
| MW | 55kDa |
| Target | GABA A Receptor |
| Entrez | 2558 |
| UniProt ID | P31644 |
| NCBI | NP_000801.1 |
| Storage | Conjugated antibodies should be stored according to the product label |
| Buffer/Preservatives | 95.64mM Phosphate, 2.48mM MES and 2mM EDTA |
| Alternative names | Gamma-aminobutyric acid receptor subunit alpha-5 a Read more... |
| Note | For research use only |
| Application notes | A 1:100 dilution of SMC-494 was sufficient for detection of GABA-A R, Alpha5 in 20 µg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. |
| Expiration Date | 12 months from date of receipt. |
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GABA A Receptor Alpha 5 Monoclonal Antibody, Clone N415/24. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-GABA A Receptor Alpha 5 Monoclonal Antibody at 1:50 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) GABA A Receptor Alpha 5 Antibody (D) Composite.
Western Blot analysis of Mouse Brain showing detection of ~55 kDa GABA A Receptor Alpha 5 protein using Mouse Anti-GABA A Receptor Alpha 5 Monoclonal Antibody, Clone N415/24. Lane 1: MW Ladder. Lane 2: Mouse Brain. Load: 20 μg. Primary Antibody: Mouse Anti-GABA A Receptor Alpha 5 Monoclonal Antibody at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Predicted/Observed Size: ~55 kDa.
