GABA A Receptor Antibody (APC)

SKU: orb376808

Description

Mouse monoclonal antibody against GABA A Receptor conjugated to APC .

Research Area

Neuroscience

Images & Validation

• Tested Applications: ICC, IF, IHC, WB
• Dilution Range: WB (1:1000), ICC/IF (1:100)
• Reactivity: Human, Mouse, Rat
• Application Notes:
  A 1:100 dilution of SMC-489 was sufficient for detection of GABA-A R, Alpha4 in 20 µg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: N398A/34 (Formerly sold as S398A-34)
• Immunogen: Fusion protein amino acids 410-450 (Cytoplasmic C-terminus) of rat GABA-A-Receptor Alpha 4
• Target: GABA A Receptor
• Molecular Weight: 60kDa
• Purification: Protein G Purified
• Conjugation: APC

Storage & Handling

• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 95.46mM Phosphate, 2.48mM MES and 2mM EDTA
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

GABA A receptor subunit alpha 4, GABRA4, Gamma aminobutyric acid A receptor Alpha 4, Gamma-aminobutyric acid receptor subunit alpha-4, GBRA4_HUMAN

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GABA-A Receptor Alpha 4 Monoclonal Antibody, Clone N398A/34. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-GABA-A Receptor Alpha 4 Monoclonal Antibody at 1:50 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) GABA-A Receptor Alpha 4 Antibody (D) Composite.

Western Blot analysis of Mouse Brain showing detection of ~60 kDa GABA-A-Receptor-Alpha4 protein using Mouse Anti-GABA-A-Receptor-Alpha4 Monoclonal Antibody, Clone N398A/34. Lane 1: MW Ladder. Lane 2: Mouse Brain. Load: 20 μg. Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-GABA-A-Receptor-Alpha4 Monoclonal Antibody at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~60 kDa.

Quick Database Links

Gene Symbol

GABA A Receptor

UniProt

P28471

NCBI Gene ID

140675

RefSeq (Protein):NP_542154.3