FZD2/Frizzled 2 Antibody

• Catalog Number: orb1538213
• Category: Antibodies
• Description: FZD2/Frizzled 2 Antibody
• Target: FZD2 / Frizzled 2
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Bovine, Canine, Equine, Hamster, Human, Monkey, Mouse, Porcine, Rat
• Concentration: 0.5 mg/ml
• Buffer/Preservatives: TBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin
• Immunogen: Peptide with sequence RYATLEHPFHCPR, from the internal region of the protein sequence according to NP_001457.1.
• Tested applications: ELISA, IF, IHC, IHC-P, WB
• Dilution range: IF (10 µg/ml), IHC, IHC-P (2.5 µg/ml), Peptide-ELISA (1:64000), WB (0.03 - 0.1 µg/ml)
• Application notes: Further information: Peptide ELISA: antibody detection limit dilution 1:64000. Western Blot: Preliminary experiments gave an approx. 23kD band in Human Colon, Heart and Ovary lysates after 0.1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 63.6kD according to NP_001457.1. The 23kD band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies / lysates? Have any further splice variants / modified forms been reported?
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: FZD2, Dfrizzled2, Fz2, Frizzled family receptor 2,
• Note: For research use only

Frizzled 2 Antibody Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).