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Catalog Number | orb1538213 |
---|---|
Category | Antibodies |
Description | FZD2/Frizzled 2 Antibody |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, IF, IHC, IHC-P, WB |
Reactivity | Bovine, Canine, Equine, Hamster, Human, Monkey, Mouse, Porcine, Rat |
Immunogen | Peptide with sequence RYATLEHPFHCPR, from the internal region of the protein sequence according to NP_001457.1. |
Concentration | 0.5 mg/ml |
Dilution range | IF (10 µg/ml), IHC, IHC-P (2.5 µg/ml), Peptide-ELISA (1:64000), WB (0.03 - 0.1 µg/ml) |
Conjugation | Unconjugated |
Target | FZD2 / Frizzled 2 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | TBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin |
Alternative names | FZD2, Dfrizzled2, Fz2, Frizzled family receptor 2, Read more... |
Note | For research use only |
Application notes | Further information: Peptide ELISA: antibody detection limit dilution 1:64000. Western Blot: Preliminary experiments gave an approx. 23kD band in Human Colon, Heart and Ovary lysates after 0.1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 63.6kD according to NP_001457.1. The 23kD band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies / lysates? Have any further splice variants / modified forms been reported? |
Expiration Date | 12 months from date of receipt. |
Frizzled 2 Antibody Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).
IHC, IHC-P | |
Bovine, Canine, Equine, Hamster, Human, Mouse, Porcine, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IHC, IHC-P | |
Bovine, Canine, Equine, Hamster, Human, Monkey, Mouse, Porcine, Rabbit, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |