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FZD2/Frizzled 2 Antibody

Catalog Number: orb1538213

DispatchUsually dispatched within 2-3 weeks
$ 670.00
Catalog Numberorb1538213
CategoryAntibodies
DescriptionFZD2/Frizzled 2 Antibody
Species/HostGoat
ClonalityPolyclonal
Tested applicationsELISA, IF, IHC, IHC-P, WB
ReactivityBovine, Canine, Equine, Hamster, Human, Monkey, Mouse, Porcine, Rat
ImmunogenPeptide with sequence RYATLEHPFHCPR, from the internal region of the protein sequence according to NP_001457.1.
Concentration0.5 mg/ml
Dilution rangeIF (10 µg/ml), IHC, IHC-P (2.5 µg/ml), Peptide-ELISA (1:64000), WB (0.03 - 0.1 µg/ml)
ConjugationUnconjugated
TargetFZD2 / Frizzled 2
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesTBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin
Alternative namesFZD2, Dfrizzled2, Fz2, Frizzled family receptor 2,
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NoteFor research use only
Application notesFurther information: Peptide ELISA: antibody detection limit dilution 1:64000. Western Blot: Preliminary experiments gave an approx. 23kD band in Human Colon, Heart and Ovary lysates after 0.1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 63.6kD according to NP_001457.1. The 23kD band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies / lysates? Have any further splice variants / modified forms been reported?
Expiration Date12 months from date of receipt.
FZD2/Frizzled 2 Antibody

Frizzled 2 Antibody Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).

  • FZD2/Frizzled 2 Antibody [orb1533976]

    IHC,  IHC-P

    Bovine, Canine, Equine, Hamster, Human, Mouse, Porcine, Rat

    Rabbit

    Polyclonal

    Unconjugated

    50 μg
  • FZD2/Frizzled 2 Antibody [orb1533977]

    IHC,  IHC-P

    Bovine, Canine, Equine, Hamster, Human, Monkey, Mouse, Porcine, Rabbit, Rat

    Rabbit

    Polyclonal

    Unconjugated

    50 μg
  • Frizzled 2 Antibody [orb1246548]

    ELISA,  IF,  IHC,  WB

    Mouse, Rat

    Human

    Goat

    Polyclonal

    Unconjugated

    0.1 mg