FZD2 Antibody

SKU: orb1246548

Description

FZD2 Antibody

Images & Validation

• Tested Applications: ELISA, IF, IHC, WB
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 60-65kDa band observed in Human Heart and Placenta lysates (calculated MW of 63.6 kDa according to NP_001457.1) An additional band of approx. 24kDa was also observed in some lysates which was successfully blocked by incubation with the immunizing peptide. Recommended concentration: 0.03-0.1ug/ml. Primary incubation 1 hour at room temperature.Immunohistochemistry:In paraffin embedded Human Thyroid Gland shows cytoplasm and membranous staining in epithelial cells of the follicles. Recommended concentration: 2-5ug/ml. Paraffin embedded Human Liver. Recommended concentration: 2.5ug/ml.Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm of U2OS cells. Recommended concentration: 10ug/ml.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: RYATLEHPFHCPR
• Target: FZD2
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Disclaimer: For research use only

Alternative Names

FZD2 , frizzled homolog 2 (Drosophila) , frizzled 2

orb1246548 (2.5 ug/ml) staining of paraffin embedded Human Thyroid Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1246548 (2.5 ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1246548 (0.03 ug/ml) staining of Human Heart (A) and (0.1 ug/ml) Placenta (B) lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1246548 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing nuclear and cytoplasmic staining.

Quick Database Links

Gene Symbol

FZD2

UniProt

Q14332

RefSeq (Protein):NP_001457.1