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Frizzled 2 Antibody

Catalog Number: orb1246548

DispatchUsually dispatched within 5-10 working days
$ 880.00
Catalog Numberorb1246548
CategoryAntibodies
DescriptionFrizzled 2 Antibody
Species/HostGoat
ClonalityPolyclonal
Tested applicationsELISA, IF, IHC, WB
Predicted ReactivityMouse, Rat
ReactivityHuman
ImmunogenThe immunogen for this antibody is: RYATLEHPFHCPR
Antibody TypePrimary Antibody
Concentration500 ug/mL
Form/AppearanceLiquid
ConjugationUnconjugated
TargetFZD2
UniProt IDQ14332
NCBINP_001457.1
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Alternative namesFZD2 , frizzled homolog 2 (Drosophila) , frizzled
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NoteFor research use only
Application notesPeptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 60-65kDa band observed in Human Heart and Placenta lysates (calculated MW of 63.6 kDa according to NP_001457.1) An additional band of approx. 24kDa was also observed in some lysates which was successfully blocked by incubation with the immunizing peptide. Recommended concentration: 0.03-0.1ug/ml. Primary incubation 1 hour at room temperature.Immunohistochemistry:In paraffin embedded Human Thyroid Gland shows cytoplasm and membranous staining in epithelial cells of the follicles. Recommended concentration: 2-5ug/ml. Paraffin embedded Human Liver. Recommended concentration: 2.5ug/ml.Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm of U2OS cells. Recommended concentration: 10ug/ml.
Expiration Date12 months from date of receipt.
Frizzled 2 Antibody

orb1246548 (2.5 ug/ml) staining of paraffin embedded Human Thyroid Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Frizzled 2 Antibody

orb1246548 (2.5 ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Frizzled 2 Antibody

orb1246548 (0.03 ug/ml) staining of Human Heart (A) and (0.1 ug/ml) Placenta (B) lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

Frizzled 2 Antibody

orb1246548 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing nuclear and cytoplasmic staining.

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