FYN Antibody

• Catalog Number: orb1927213
• Category: Antibodies
• Description: Purified Mouse Monoclonal Antibody (Mab)
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 1302CT390.118.237
• Tested applications: FC, IHC-P, WB
• Reactivity: Human
• Isotype: IgG1,κ
• Dilution range: WB: 1:1000, IHC-P: 1:25, IHC-P: 1:25, IHC-P: 1:25, FC: 1:25
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 60762 Da
• Target: This FYN antibody is generated from a mouse immunized with a recombinant preotein from human FYN.
• UniProt ID: P06241
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Tyrosine-protein kinase Fyn, Proto-oncogene Syn, P
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of lysate from 293 cell line using FYN. Diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L (HRP) at 1: 3000 dilution was used as the secondary antibody. Lysate at 35μg per lane.

Flow cytometric analysis of Hela cells using FYN (green) compared to an isotype control of mouse IgG1 (blue). Diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.

Immunohistochemical analysis of paraffin-embedded R. spleen section using FYN. Diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Immunohistochemical analysis of paraffin-embedded M. spleen section using FYN. Diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Immunohistochemical analysis of paraffin-embedded H. tonsil section using FYN. Diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.