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FXR1 Antibody

Catalog Number: orb1247974

DispatchUsually dispatched within 5-10 working days
$ 880.00
Catalog Numberorb1247974
CategoryAntibodies
DescriptionFXR1 Antibody
Species/HostGoat
ClonalityPolyclonal
Tested applicationsELISA, FC, IF, WB
Predicted ReactivityBovine, Canine, Rat
ReactivityHuman, Mouse
ImmunogenThe immunogen for this antibody is: C-RIEGDNENKLPRED
Antibody TypePrimary Antibody
Concentration500 ug/mL
Form/AppearanceLiquid
ConjugationUnconjugated
TargetFXR1
UniProt IDP51114
NCBINP_005078.2, NP_001013456.1, NP_001013457.1
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Alternative namesFXR1, fragile X mental retardation, autosomal homo
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NoteFor research use only
Application notesPeptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 75+80kDa band observed in lysates of cell line NIH-3T3 (calculated MW of 59.9kDa according to NP_001013457.1). The observed molecular weight corresponds to earlier findings in literature (Khandjian et al Hum Mol Genet. 1998 Dec;7(13):2121-8.; PMID: 9817930). An additional band of unknown identity was also consistently observed at 37kDa. This band was successfully blocked by incubation with the immunizing peptide. Recommended concentration. 1-3ug/ml. Primary incubation 1 hour at room temperature.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A431 and HeLa cells. Recommended concentration: 10ug/ml. Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.
Expiration Date12 months from date of receipt.
FXR1 Antibody

orb1247974 (1 ug/ml) staining of NIH-3T3 cell lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

FXR1 Antibody

orb1247974 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI.

FXR1 Antibody

orb1247974 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI.

FXR1 Antibody

orb1247974 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line).

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