FXR1 Antibody

• Catalog Number: orb19295
• Category: Antibodies
• Description: Goat polyclonal antibody to FXR1
• Target: FXR1
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Canine, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: RIEGDNENKLPRED
• RRID: AB_10750673
• MW: 69.7; 60.8; 59.9
• Tested applications: ELISA, FC, IF, WB
• Dilution range: ELISA: 1:128000, WB: 1-3 μg/ml, IHC-P: 10ug/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx 80kDa band observed in lysates of cell line NIH/3T3 (calculated MW of 59.9kDa according to NP_001013457.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Khandjian et al Hum Mol Genet. 1998 Dec;7(13):2121-8.; PMID: 9817930). Recommended concentration: 0.1-0.3 μg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti FXR1 antibody, anti fragile X mental retardat
• Research Area: Developmental Biology, Epigenetics
• Note: For research use only
• Entrez: 8087

orb19295 (0.2ug/ml) staining of NIH/3T3 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

WB analysis of NIH-3T3 cell lysate using FXR1 antibody

Immunofluorescence analysis of A431 cells using FXR1 antibody

Immunofluorescence analysis of HeLa cells using FXR1 antibody

Flow cytometric analysis of HeLa cells using FXR1 antibody