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FUNDC1 Rabbit Polyclonal Antibody

SKU: orb156964

Description

FUNDC1 Rabbit Polyclonal Antibody

Images & Validation

Tested ApplicationsWB
Dilution RangeWB=1:300-800
ReactivityHuman, Mouse, Rat
Predicted ReactivityHuman, Rat

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenKLH conjugated synthetic peptide derived from mouse FUNDC1
TargetFUNDC1
Molecular Weight17 kDa
PurificationAffinity purified by Protein A
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

FUN14 domain containing protein 1; FUN14 domain-containing protein 1; FUND1_HUMAN.

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FUNDC1 Rabbit Polyclonal Antibody

Sample: Lane 1: Mouse Cerebellum tissue lysates, Lane 2: Mouse Heart tissue lysates, Lane 3: Human 293T cell lysates, Lane 4: Human MCF-7 cell lysates, Lane 5: Human A549 cell lysates, Primary: Anti-FUNDC1 (orb156964) at 1/800 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 17 kDa, Observed band size: 17 kDa.

UniProt Details

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol

FUNDC1 Rabbit Polyclonal Antibody (orb156964)

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Applications: WB|Reactivity: Mouse

Our lab generates a lot of protein knockouts in mouse embryonic stem cells using crispr-cas9 technology. We use Western Blots and subsequent sequencing to screen potential knockout clones. Not bad for screening knockouts, but there is a nonspecific band close to the expected band which complicates analysis. I think the lower band is the correct band, though I can't be sure.

Tamanna Hossin
- Tamanna HossinYale, Tech / Genetics

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50 μl
$ 250.00
100 μl
$ 390.00
DispatchUsually dispatched within 5-10 working days
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