FH Antibody

SKU: orb1247472

Description

FH Antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: EIA, ELISA, IHC, WB
• Reactivity: Human
• Predicted Reactivity: Canine, Mouse, Rat
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 48kDa band observed in Human Kidney, Liver and Lung lysates (calculated MW of 54.6kDa according to NP_000134.2). The observed molecular weight corresponds to earlier findings with antibodies from different sources. In transfected HEK293 transiently expressing Fumarase a band of approx. 55kDa is observed. This band is not observed in the non-transfected HEK293.Recommended concentration: 0.01-0.03µg/mL.Immunohistochemistry:Paraffin embedded Human Skeletal Muscle and Heart. Recommended concentration: 3.75µg/mL.Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant Fumarase captured by a rabbit antibody. Recommended reporter concentration: 1-2µg/mL

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Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-HPNDHVNKSQSSND
• Target: FH
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

fumarate hydratase, HLRCC, LRCC, MCL, MCUL1, fumarase, leiomyomatosis and renal cell cancer, multiple hereditary cutaneous leiomyomata

Quick Database Links

Gene Symbol

FH

UniProt

P07954

RefSeq (Protein):NP_000134.2