LDLR Rabbit Polyclonal Antibody

• Catalog Number: orb10972
• Category: Antibodies
• Description: LDLR Rabbit Polyclonal Antibody
• Target: LDLR
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Canine, Equine, Guinea pig, Porcine, Rabbit, Rat
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Immunogen: KLH conjugated synthetic peptide derived from human LDL-R (781-860/860aa)
• UniProt ID: P01130
• RRID: AB_10750179
• MW: 92 kDa
• Tested applications: FC, ICC, WB
• Dilution range: WB=1:500-2000, ICC/IF=1:100, Flow-Cyt=1μg/Test
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: LDLR_HUMAN; Low-density lipoprotein receptor; LDL
• Note: For research use only

Blank control (black line): HepG2 (black) (The cells were fixed with 2% paraformaldehyde (10 min), then permeabilized with PBST for 30 min on room temperature), Primary Antibody (green line): Rabbit Anti-LDLreceptor antibody (orb10972), Dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, Dilution: 1 µg/Test.

Blank control: A431. Primary Antibody (green line): Rabbit Anti-LDL receptor antibody (orb10972), Dilution: 1 µg/10^6 cells. Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody:Goat anti-rabbit IgG-AF647, Dilution: 1 µg/Test. Protocol, A431 cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Blank control: Raw264.7. Primary Antibody (green line): Rabbit Anti-LDL receptor antibody (orb10972), Dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF647, Dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

HepG2 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (LDL receptor) polyclonal Antibody, Unconjugated (orb10972) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Sample: Human colon Lysate at 45ug, Primary: Anti-LDL receptor (orb10972) at 1:300 dilution, Secondary: HRP conjugated Goat Anti-Rabbit IgG (orb572747) at 1:5000 dilution, Predicted band size: 92kD, Observed band size: 92kD.

Sample: K562 (Human) Cell Lysate at 30 ug, Raw264.7 (Mouse) Cell Lysate at 30 ug, HepG2 (Human) Cell Lysate at 30 ug, SW480 (Human) Cell Lysate at 30 ug, Primary: Anti-LDL receptor (orb10972) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 92 kD, Observed band size: 120 kD.