FOXC2 Antibody

• Catalog Number: orb1248892
• Category: Antibodies
• Description: FOXC2 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, FC, WB
• Predicted Reactivity: Bovine, Canine, Rat
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: RHAAPYSYDCTKY
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 35kDa band observed in lysates of cell lines K562 and HeLa, which was successfully blocked by incubation with the immunizing peptide (calculated MW of 38.8kDa according to Human NP_075555.1). Recommended concentration 1-2ug/ml. Primary incubation 1 hour at room temperature.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: FOXC2
• UniProt ID: Q99958
• NCBI: NP_005242.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: FOXC2, forkhead box C2 (MFH-1, mesenchyme forkhead
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 35kDa band observed in lysates of cell lines K562 and HeLa, which was successfully blocked by incubation with the immunizing peptide (calculated MW of 38.8kDa according to Human NP_075555.1). Recommended concentration 1-2ug/ml. Primary incubation 1 hour at room temperature.
• Expiration Date: 12 months from date of receipt.

orb1248892 staining (2 μg/ml) of nuclear HEK293 (A) and (1 ug/ml) negative control Human Pancreas (B) lysate [RIPA buffer, 35 μg total protein per lane). Detected by chemiluminescence.

orb1248892 (2 ug/ml) staining of paraffin embedded Human Breast.

orb1248892 Flow cytometric analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.