FOXC2 Antibody

SKU: orb1248892

Description

FOXC2 Antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: ELISA, FC, WB
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Rat
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 35kDa band observed in lysates of cell lines K562 and HeLa, which was successfully blocked by incubation with the immunizing peptide (calculated MW of 38.8kDa according to Human NP_075555.1). Recommended concentration 1-2µg/mL. Primary incubation 1 hour at room temperature.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: RHAAPYSYDCTKY
• Target: FOXC2
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

FOXC2, forkhead box C2 (MFH-1, mesenchyme forkhead 1), MFH1, MFH-1, FKHL14, MFH-1,mesenchyme forkhead 1, forkhead (Drosophila)-like 14, forkhead, Drosophila, homolog-like 14, forkhead (Drosophila)-like 14 (MFH-1, mesenchyme forkhead 1), LD, forkhead box C2, forkhead, Drosophila, homolog-like 14

orb1248892 staining (2 μg/ml) of nuclear HEK293 (A) and (1 ug/ml) negative control Human Pancreas (B) lysate [RIPA buffer, 35 μg total protein per lane). Detected by chemiluminescence.

orb1248892 (2 ug/ml) staining of paraffin embedded Human Breast.

orb1248892 Flow cytometric analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Quick Database Links

Gene Symbol

FOXC2

UniProt

Q99958

RefSeq (Protein):NP_005242.1