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| Catalog Number | orb18538 |
|---|---|
| Category | Antibodies |
| Description | Goat polyclonal antibody to FOXC2 |
| Target | FOXC2 |
| Clonality | Polyclonal |
| Species/Host | Goat |
| Conjugation | Unconjugated |
| Reactivity | Human |
| Predicted Reactivity | Bovine, Canine, Rat |
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Protein Sequence | RHAAPYSYDCTKY |
| RRID | AB_10749075 |
| MW | 53.7 |
| Tested applications | ELISA, FC, WB |
| Dilution range | ELISA: 1:4000, WB: 2 μg/ml, IHC-P: 10ug/ml |
| Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx 50-55kDa band observed in Human Breast Cancer lysates (calculated MW of 53.7kDa according to NP_005242.1). Recommended concentration: 1-3 μg/ml. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | anti FOXC2 antibody, anti forkhead box C2 (MFH-1, Read more... |
| Research Area | Developmental Biology, Epigenetics |
| Note | For research use only |
| Entrez | 2303 |

Western blot analysis of Human Breast Cancer lysate using FOXC2 antibody

Immunohistochemical staining of Human Breast cancer using FOXC2 antibody

Western blot analysis of HEK293 lysate (10ug protein in RIPA buffer) using FOXC2 antibody

Immunohistochemical analysis of formalin-fixed and paraffin embedded Human Breast cancer tissue using FOXC2 antibody

Flow cytometric analysis of HEK293 cells using FOXC2 antibody

Western blot analysis of nuclear HEK293 (A) and Human Pancreas (B) lysate using FOXC2 antibody
ELISA, IF, IHC-P, WB | |
Human, Mouse, Rat | |
Polyclonal | |
Unconjugated |
Human | |
0.32-20 ng/mL | |
0.137 ng/mL |
FC, IHC-P, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
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