FOXA1 Antibody

SKU: orb750046

Description

The transcription factor Forkhead-box A1 (FOXA1), also known as hepatocyte nuclear factor 3-alpha, is a member of the FOX class of transcription factors. HNF-1 (α and β), HNF-3 (α, β and γ), HNF-4 (α and γ), and HNF-6 compose, in part, a homeoprotein family designated the hepatocyte nuclear factor family. The various HNF-1 isoforms regulate transcription of genes in the liver as well as in other tissues such as kidney, small intestine and thymus. FOXA1 is expressed in normal breast ductal epithelium and other epithelium in different organs, such as lung, pancreas, bladder, prostate, and colon. Recently, FOXA1 has been shown to be a major determinant of estrogen-ER activity and endocrine response in breast cancer cells. FOXA1 expression correlates with estrogen receptor (ER)-positivity, especially in luminal subtype A breast cancers, which is associated with favorable prognosis. FOXA1 is useful in the sub-classification of breast carcinomas.

Research Area

Developmental Biology, Epigenetics

Images & Validation

• Tested Applications: FACS, IF, IHC-P, WB
• Dilution range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Immunofluorescence: 2-4ug/ml,Flow cytometry: 1-2ug/million cells
• Reactivity: Human, Rat
• Application Notes:
  The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the FOXA1 antibody to be titered up or down for optimal performance.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2a, kappa
• Clone No.: FOXA1/1519
• Immunogen: A human partial recombinant protein corresponding to amino acids 372-472 was used as the immunogen for this FOXA1 antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 1X PBS, pH 7.4
• Disclaimer: For research use only

IHC testing of FFPE human prostate carcinoma with FOXA1 antibody (clone FOXA1/1519). HIER: boil sections in 10mM Tris with 1mM EDTA, pH9, for 10-20 min.

IHC testing of FFPE rat colon with FOXA1 antibody (clone FOXA1/1519). HIER: boil sections in 10mM Tris with 1mM EDTA, pH9, for 10-20 min.

Immunofluorescent staining of PFA-fixed human MCF7 cells with FOXA1 antibody (clone FOXA1/1519, green) and Phalloiden (red).

Western blot testing of human HeLa cell lysate with FOXA1 antibody (clone FOXA1/1519). Predicted molecular weight: ~49 kDa.

FACS testing of PFA-fixed human MCF-7 cells with FOXA1 antibody (clone FOXA1/1519); Red = isotype control, Blue = FOXA1 antibody.

SDS-PAGE Analysis of Purified, BSA-Free FOXA1 Antibody (clone FOXA1/1519). Confirmation of Integrity and Purity of the Antibody.

Quick Database Links

UniProt

P55317