FOXA1/HNF3A Antibody

SKU: orb18549

Description

Goat polyclonal antibody to FOXA1

Research Area

Developmental Biology, Epigenetics

Images & Validation

• Tested Applications: ELISA, FC, IF, WB
• Dilution range: ELISA: 1:128000, WB: 0.1-0.3 μg/ml, IHC-P: 10ug/ml, FACS: 10ug/ml
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Porcine, Rat
• Application Notes:
  WB: Approx 50kDa band observed in nuclear lysates of cell line HepG2 and approx 50-55kDa in Mouse Liver and in nuclear lysates of cell line MCF7 calculated MW of 49.1kDa according to Human NP_004487.2 and 48.9kDa according to Mouse NP_032285.2).These bands we. Notes: Immunofluorescence: Strong expression of the protein seen in the nuclei of MCF7 and U2OS cells. Recommended concentration: 10µg/ml. Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recommended concentration: 10u

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: FOXA1 / HNF3A
• Molecular Weight: 49.1
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti FOXA1 antibody, anti HNF3A antibody, anti forkhead box A1 antibody, anti TCF3A antibody, anti MGC33105 antibody, anti hepatocyte nuclear factor 3, alpha antibody

Immunohistochemical staining of Human Prostate using FOXA1 antibody

Immunohistochemical staining of Human Breast using FOXA1 antibody

Western blot analysis of HepG2 (A) and (0.3ug/ml) MCF7 (B) nuclear cell lysate using FOXA1 antibody.

Western blot analysis of Mouse Liver lysate using FOXA1 antibody.

Immunofluorescence analysis of MCF7 cells using FOXA1 antibody.

Immunofluorescence analysis of U2OS cells using FOXA1 antibody.

Flow cytometric analysis of MCF7 cells using FOXA1 antibody.

Quick Database Links

Gene Symbol

FOXA1 / HNF3A

NCBI Gene ID

3169