FOXA1 Antibody

SKU: orb1252290

Description

FOXA1 Antibody

Images & Validation

• Tested Applications: FC, IF, IHC, WB
• Reactivity: Human
• Application Notes:
  Flow Cytometry: 0.5-1ug/10^6 cellsIF: 1-2 µg/mLWB: 0.5-1 µg/mLIHC (FFPE): 1-2 µg/mL for 30 min at RTOptimal dilution of the FOXA1 antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone No.: FOXA1/1512
• Immunogen: A human partial recombinant protein corresponding to amino acids 372-472 was used as the immunogen for this FOXA1 antibody.
• Target: FOXA1
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Concentration: 0.2 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

FOXA1 Antibody: HNF3A, TCF3A, HNF3A, Hepatocyte nuclear factor 3-alpha, Forkhead box protein A1, HNF-3-alpha

IHC testing of FFPE human prostate carcinoma with FOXA1 antibody (clone FOXA1/1512). HIER: boil sections in 10mM Tris with 1mM EDTA, pH9, for 10-20 min.

IHC testing of FFPE human prostate with FOXA1 antibody (clone FOXA1/1512). HIER: boil sections in 10mM Tris with 1mM EDTA, pH9, for 10-20 min.

Western blot testing of human 1) MCF7 (breast) and 2) PC3 (prostate) cell lysate with FOXA1 antibody (clone FOXA1/1512). Predicted molecular weight: ~49 kDa.

SDS-PAGE Analysis of Purified, BSA-Free FOXA1 Antibody (clone FOXA1/1512). Confirmation of Integrity and Purity of the Antibody.

Quick Database Links

Gene Symbol

FOXA1

UniProt

P55317