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Catalog Number | orb1926343 |
---|---|
Category | Antibodies |
Description | Purified Rabbit Polyclonal Antibody (Pab) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Clone Number | RB53014 |
Tested applications | IHC-P, WB |
Reactivity | Human, Mouse |
Isotype | Rabbit IgG |
Dilution range | WB: 1:1000-1:2000, IHC-P: 1:25 |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Conjugation | Unconjugated |
MW | 34412 Da |
Target | This FN3KRP antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 24-58 amino acids from human FN3KRP. |
UniProt ID | Q9HA64 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | Ketosamine-3-kinase, 271-, Fructosamine-3-kinase-r Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
All lanes: Anti-FN3KRP Antibody (N-Term) at 1:1000-1:2000 dilution. Lane 1: U-87 MG whole cell lysates. Lane 2: 293T-17 whole cell lysates. Lane 3: NCI-H1299 whole cell lysates. Lane 4: Jurkat whole cell lysates. Lane 5: mouse kidney lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 34 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Staining FN3KRP in human Stomach sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.