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Description
Research Area
Images & Validation
−| Tested Applications | IHC, WB |
|---|---|
| Dilution Range | WB: 1:2000, IHC: 1:25 |
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Immunogen | This FN3KRP antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 24-58 amino acids from human FN3KRP. Antigen Region: 24-58 aa. |
| Target | FN3KRP {ECO:0000303|PubMed:15137908, ECO:0000312|HGNC:HGNC:25700} |
| Molecular Weight | 34412 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−FN3KRP Antibody (N-Term) [orb1926343]
IHC-P, WB
Human, Mouse
Rabbit
Polyclonal
Unconjugated
100 μl, 50 μl

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All lanes: Anti-FN3KRP Antibody (N-Term) at 1:2000 dilution. Lane 1: 293T/17 whole cell lysate. Lane 2: Jurkat whole cell lysate. Lane 3: NCI-H1299 whole cell lysate. Lane 4: U-87 MG whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 34 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Staining FN3KRP in human Stomach sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
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Protocol Information
FN3KRP Antibody (N-Term) (orb1787943)
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