FMR1 Antibody

SKU: orb1246550

Description

FMR1 Antibody

Research Area

Neuroscience

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Human
• Predicted Reactivity: Canine, Mouse, Rat
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 80kDa band observed in lysates of cell line KELLY (calculated MW of 71.2kDa according to NP_002015.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Sittler et al, Hum Mol Genet. 1996 Jan;5(1):95-102; PMID: 8789445). Recommended concentration: 0.1-0.3µg/mL. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Placenta. Recommended concentration: 2.5µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-RTGKDRNQKKEKPD
• Target: FMR1
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

FMR1, fragile X mental retardation 1 , FMRP, FRAXA, MGC87458

orb1246550 (0.1 ug/ml) staining of KELLY cell lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1246550 (2.5 ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

FMR1

UniProt

Q06787

RefSeq (Protein):NP_002015.1