FMR1 Antibody

SKU: orb19826

Description

Goat polyclonal antibody to FMR1

Research Area

Neuroscience

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:128000, WB: 0.1-0.3 μg/ml, IHC-P: 2.5 μg/ml
• Reactivity: Human
• Predicted Reactivity: Canine, Mouse, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx 80kDa band observed in lysates of cell line KELLY (calculated MW of 71.2kDa according to NP_002015.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Sittler et al, Hum Mol Genet. 1996 Jan;5(1):95-102; PMID: 8789445). Recommended concentration: 0.1-0.3 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: FMR1
• Protein Sequence: RTGKDRNQKKEKPD
• Molecular Weight: 71.2
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti FMR1 antibody, anti fragile X mental retardation 1 antibody, anti FMRP antibody, anti FRAXA antibody, anti MGC87458 antibody

Western blot analysis of KELLY cell lysate using FMR1 antibody

Immunohistochemical staining of Human Placenta using FMR1 antibody

Quick Database Links

Gene Symbol

FMR1

NCBI Gene ID

2332