FLI1 Antibody

• Catalog Number: orb1257402
• Category: Antibodies
• Description: FLI1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human FLI1 (NP_002008.2).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IP: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 53kDa
• Target: FLI1
• UniProt ID: Q01543
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: FLI1 Antibody: EWSR2, SIC-1, BDPLT21
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IP: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of Jurkat cells, using FLI1 antibody (orb1257402) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded human lung cancer using FLI1 antibody (orb1257402) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human uterine cancer using FLI1 antibody (orb1257402) at dilution of 1:100 (40x lens).

Immunoprecipitation analysis of 200 ug extracts of Jurkat cells using 1 ug FLI1 antibody (orb1257402). Western blot was performed from the immunoprecipitate using FLI1 antibody (orb1257402) at a dilution of 1:1000.