FLI1 Antibody

SKU: orb1257402

Description

FLI1 Antibody

Research Area

Musculoskeletal & Connective Tissue Research

Images & Validation

• Tested Applications: IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:50 - 1:200IP: 1:50 - 1:200

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human FLI1 (NP_002008.2).
• Target: FLI1
• Molecular Weight: Observed: 53kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

FLI1 Antibody: EWSR2, SIC-1, BDPLT21

Western blot analysis of extracts of Jurkat cells, using FLI1 antibody (orb1257402) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded human lung cancer using FLI1 antibody (orb1257402) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human uterine cancer using FLI1 antibody (orb1257402) at dilution of 1:100 (40x lens).

Immunoprecipitation analysis of 200 ug extracts of Jurkat cells using 1 ug FLI1 antibody (orb1257402). Western blot was performed from the immunoprecipitate using FLI1 antibody (orb1257402) at a dilution of 1:1000.

Quick Database Links

Gene Symbol

FLI1

UniProt

Q01543