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EWS/EWSR1 Antibody

SKU: orb20151

Description

Goat polyclonal antibody to EWSR1

Images & Validation

Tested ApplicationsELISA, IHC, WB
Dilution rangeELISA: 1:64000, WB: 0.3-1 μg/ml
ReactivityHuman
Predicted ReactivityBovine, Mouse, Porcine, Rat
Application Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx. 90kDa band observed in Nuclear lysates of cell line HeLa (calculated MW of 68.5kDa according to NP_053733.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 1-3 μg/ml.

Key Properties

HostGoat
ClonalityPolyclonal
TargetEWS / EWSR1
Molecular Weight69.0; 68.5; 68.4; 62.5; 37.6
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
DisclaimerFor research use only

Alternative Names

anti EWSR1 antibody, anti EWS antibody, anti Ewing sarcoma breakpoint region 1 antibody, anti EWS/FLI fusion protein antibody, anti EWSR1/ZNF384 fusion protein antibody, anti Ewings sarcoma EWS-Fli1 (type 1) oncogene antibody, anti bK984G1.4 (Ewing sarcoma breakpoint region 1 protein) antibody, anti extraskeletal myxoid chondrosarcoma EWS/TEC/CHN fusion protein antibody

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EWS/EWSR1 Antibody

Western blot analysis of nuclear HeLa lysate using EWSR1 antibody

EWS/EWSR1 Antibody

Western blot analysis of HeLa lysate using EWSR1 antibody

EWS/EWSR1 Antibody

Immunohistochemical staining of Human Small Intestine using EWSR1 antibody

EWS/EWSR1 Antibody

Immunohistochemical staining of Human Uterus using EWSR1 antibody

NCBI Gene Details

No NCBI Gene data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

EWS/EWSR1 Antibody (orb20151)

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Available Sizes

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100 μg
$ 500.00
DispatchUsually dispatched within 5-10 working days
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