Flagellin Antibody

SKU: orb344675

Description

Flagellin antibody

Images & Validation

• Tested Applications: ELISA, WB
• Dilution range: ELISA: 1:6,000, WB: 1:1,000
• Reactivity: Bacteria
• Application Notes:
  This protein-A purified antibody has been tested for use in ELISA and Western blotting. Specific conditions for reactivity should be optimized by the user. Expect a band approximately 33.9 kDa in size corresponding to Borrelia burgdorferi Flagellin protein by Western blotting in the appropriate cell lysate or extract.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: MBP-fusion protein corresponding to Borrelia burgdorferi Flagellin protein.
• Purity: This product was Protein-A purified and cross-adsorbed against MBP from monospecific antiserum by chromatography. This antibody is specific for Lyme Borrelia spp. Flagellin protein. A BLAST analysis was used to suggest cross-reactivity with Flagellin from B. burgdorferi, garinii, and valaisiana sources based on 100% homology with the immunizing sequence.. Cross-reactivity with Flagellin from other sources has not been determined.

Storage & Handling

• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Concentration: 1.0 mg/mL
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-Flagellin Antibody, 41 kDa antigen, Borrelia burgdorferi p41, fla, Flagellar filament 41 kDa core protein, Bacterial flagellin

Western Blot of Anti-Flagellin Antibody. Lane 1: Flagellin Control Protein (p/n orb345967) Reduced [0.1 µg]. Lane 2: Opal Prestained Molecular Weight Markers. Lane 3: Flagellin Control Protein Non-Reduced [0.1 µg]. Primary Antibody: Anti-Flagellin at 1.0 µg/ml overnight at 4°C. Secondary Antibody: Goat anti-Rabbit IgG peroxidase (p/n orb347654) at 1:70000 for 30 mins at RT. Block: BLockOut (p/n orb348644) for 1hrs at RT. Predicted MW: ~76.3kDa. Exposure: 2sec.

Western blot showing detection of 0.1 µg of recombinant Flagellin protein. Lane 1: Molecular weight markers. Lane 2: MBP-Flagellin fusion protein (arrowhead at expected MW: 76.3 kDa). Lane 3: MBP alone. Protein was run on a 4-20% gel, then transferred to 0.45 µm nitrocellulose. After blocking with 1% BSA-TTBS (p/n orb348540, diluted to 1X) overnight at 4°C, primary antibody was used at 1:1000 at room temperature for 30 min. HRP-conjugated Goat-Anti-Rabbit (p/n orb347654) secondary antibody was used at 1:40000 in orb348637 blocking buffer.

Quick Database Links

UniProt

P11089

RefSeq (Protein):WP_002661938.1