Flagellin Antibody

SKU: orb344674

Description

Flagellin antibody

Images & Validation

• Tested Applications: ELISA, WB
• Dilution range: ELISA: 1:6,000, WB: 1:1,000
• Reactivity: Bacteria
• Application Notes:
  This protein-A purified antibody has been tested for use in ELISA and Western blotting. Specific conditions for reactivity should be optimized by the user. Expect a band approximately 33.9 kDa in size corresponding to Borrelia burgdorferi Flagellin protein by Western blotting in the appropriate cell lysate or extract.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: MBP-fusion protein corresponding to Borrelia burgdorferi Flagellin protein.
• Purity: This product was Protein-A purified and cross-adsorbed against MBP from monospecific antiserum by chromatography. This antibody is specific for Lyme Borrelia spp. Flagellin protein. A BLAST analysis was used to suggest cross-reactivity with Flagellin from B. burgdorferi, garinii, and valaisiana sources based on 100% homology with the immunizing sequence.. Cross-reactivity with Flagellin from other sources has not been determined.

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Concentration: 1.2 mg/ml
• Disclaimer: For research use only

Alternative Names

rabbit anti-Flagellin Antibody, 41 kDa antigen, Borrelia burgdorferi p41, fla, Flagellar filament 41 kDa core protein, Bacterial flagellin.

Western Blot of Anti-Flagellin Antibody. Lane 1: Flagellin Control Protein (p/n orb345967) Reduced [0.1 µg]. Lane 2: Opal Prestained Molecular Weight Markers. Lane 3: Flagellin Control Protein Non-Reduced [0.1 µg]. Primary Antibody: Anti-Flagellin at 1.0 µg/ml overnight at 4°C. Secondary Antibody: Goat anti-Rabbit IgG peroxidase (p/n orb347654) at 1:70000 for 30 mins at RT. Block: BLockOut (p/n orb348644) for 1hrs at RT. Predicted MW: ~76.3kDa. Exposure: 2sec.

Western blot showing detection of 0.1 µg of recombinant Flagellin protein. Lane 1: Molecular weight markers. Lane 2: MBP-Flagellin fusion protein (arrowhead at expected MW: 76.3 kDa). Lane 3: MBP alone. Protein was run on a 4-20% gel, then transferred to 0.45 µm nitrocellulose. After blocking with 1% BSA-TTBS (p/n orb348540, diluted to 1X) overnight at 4°C, primary antibody was used at 1:1000 at room temperature for 30 min. HRP-conjugated Goat-Anti-Rabbit (p/n orb347654) secondary antibody was used at 1:40000 in orb348637 blocking buffer.

Quick Database Links

UniProt

P11089

RefSeq (Protein):WP_002661938.1