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Catalog Number | orb344674 |
---|---|
Category | Antibodies |
Description | Flagellin antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, WB |
Reactivity | Bacteria |
Isotype | IgG |
Immunogen | MBP-fusion protein corresponding to Borrelia burgdorferi Flagellin protein. |
Concentration | 1.2 mg/ml |
Dilution range | ELISA: 1:6,000, WB: 1:1,000 |
Form/Appearance | Lyophilized |
Purity | This product was Protein-A purified and cross-adsorbed against MBP from monospecific antiserum by chromatography. This antibody is specific for Lyme Borrelia spp. Flagellin protein. A BLAST analysis was used to suggest cross-reactivity with Flagellin from B. burgdorferi, garinii, and valaisiana sources based on 100% homology with the immunizing sequence.. Cross-reactivity with Flagellin from other sources has not been determined. |
Conjugation | Unconjugated |
UniProt ID | P11089 |
NCBI | ZP_03087263.1 |
Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | rabbit anti-Flagellin Antibody, 41 kDa antigen, Bo Read more... |
Note | For research use only |
Application notes | This protein-A purified antibody has been tested for use in ELISA and Western blotting. Specific conditions for reactivity should be optimized by the user. Expect a band approximately 33.9 kDa in size corresponding to Borrelia burgdorferi Flagellin protein by Western blotting in the appropriate cell lysate or extract. |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of recombinant Flagellin protein. Lane 1: Molecular weight markers. Lane 2: MBP-Flagellin fusion protein (arrowhead at expected MW: 76.3 kDa). Lane 3: MBP alone using Flagellin antibody
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