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Catalog Number | orb1562810 |
---|---|
Category | Antibodies |
Description | Flag Tag Mouse mAb |
Clonality | Monoclonal |
Isotype | IgG1 |
Conjugation | Unconjugated |
Reactivity | Other |
Form/Appearance | Liquid |
Concentration | 2.3 mg/ml |
Buffer/Preservatives | Liquid in PBS containing 50% glycerol, 0.5% rAlbumin and 0.02% sodium azide, pH 7.3. |
Purification | Affinity Purified |
Immunogen | DYKDDDDK synthetic peptide conjugated to KLH. |
Tested applications | IP, WB |
Dilution range | WB: 1/1000-1/10000 IP: 1/20 |
Clone Number | 3A6-E5-C10 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | DYKDDDDK Tag; Flag; FLAG |
Note | For research use only |
Western blot analysis of Flag Tag in CHO-K1 (C) or CHO-K1 transfected with different FLAGfusion proteins (A, B), using FlagTag antibody.
Western blot analysis of Flag Tag in CHO-K1 cells transfected with Flag-GADD45α (C and D), 3*Flag-GADD45α (A and B) as indicated, using Flagtag antibody (left) and GADD45α antibody (orb1563304, right) as loading control.