Flag Tag (7E1) Mouse mAb Antibody

SKU: orb1562815

Description

Flag Tag (7E1) Mouse mAb Antibody

Research Area

Disease Biomarkers

Images & Validation

• Tested Applications: ICC, IP, WB
• Dilution Range: WB: 1/1000-1/10000 IF: 1/100-1/500 IP: 1/20
• Reactivity: Other

Key Properties

• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: 7E1-10D2-3H1
• Immunogen: Synthetic Peptide of Flag-Tag
• Purification: Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Liquid in PBS containing 50% glycerol, 0.5% rAlbumin and 0.02% sodium azide, pH 7.3.
• Concentration: 1.3 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

DYKDDDDK Tag; Flag; FLAG

Immunofluorescence analysis of Flag Tag (7E1) ) in 293 cells transfected with a Flagtag protein using Flag Tag (7E1) antibody (red), and DAPI (blue).

Immunoprecipitation analysis of Flag Tag (7E1) in 1) untransfected 293 cell lysates 2) transfected 293 cell lysates with Flagtag fusion protein 3) IP (transfected 293+ normal mouse IgG+Protein G agarose) 4) IP (transfected 293+anti Flag mA+Protein G agarose).

Western blot analysis of Flag Tag (7E1) in CHO-K1 cells (C) or CHO-K1 cells transfected with different FLAGfusion proteins (A, B), using FlagTag antibody.