Flag Tag (7E1) Mouse mAb

• Catalog Number: orb1562815
• Category: Antibodies
• Description: Flag Tag (7E1) Mouse mAb
• Clonality: Monoclonal
• Isotype: IgG1
• Conjugation: Unconjugated
• Reactivity: Other
• Form/Appearance: Liquid
• Concentration: 1.3 mg/ml
• Buffer/Preservatives: Liquid in PBS containing 50% glycerol, 0.5% rAlbumin and 0.02% sodium azide, pH 7.3.
• Purification: Affinity Purified
• Immunogen: Synthetic Peptide of Flag-Tag
• Tested applications: ICC, IF, IP, WB
• Dilution range: WB: 1/1000-1/10000 IF: 1/100-1/500 IP: 1/20
• Clone Number: 7E1-10D2-3H1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: DYKDDDDK Tag; Flag; FLAG
• Research Area: Disease Biomarkers
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence analysis of Flag Tag (7E1) ) in 293 cells transfected with a Flagtag protein using Flag Tag (7E1) antibody (red), and DAPI (blue).

Immunoprecipitation analysis of Flag Tag (7E1) in 1) untransfected 293 cell lysates 2) transfected 293 cell lysates with Flagtag fusion protein 3) IP (transfected 293+ normal mouse IgG+Protein G agarose) 4) IP (transfected 293+anti Flag mA+Protein G agarose).

Western blot analysis of Flag Tag (7E1) in CHO-K1 cells (C) or CHO-K1 cells transfected with different FLAGfusion proteins (A, B), using FlagTag antibody.