Fibrillarin (Nop1p) Antibody

SKU: orb3167402

Description

Our Anti-rRNA 2'O-methyltransferase fibrillarin (Nop1p) mouse monoclonal primary antibody detects C. elegans, drosophila, human, other mammals (predicted), rat, and yeast rRNA 2'O-methyltransferase fibrillarin (Nop1p), and is IgG. It is validated for use in ICC, WB.

Images & Validation

• Tested Applications: ICC, WB
• Dilution Range: WB: 1:500-1:10000 (see notes); ICC: 1:500-1:5000 (see notes)
• Reactivity: Drosophila, Human, Rat, Yeast
• Predicted Reactivity: Mammal
• Application Notes:
  A dilution of 1:2000 (cell lysates) to 1:10000 (nuclear fractions) is recommended for WB of yeast protein samples followed by chemiluminescent detection (ECL). For other non-ECL WB methods, a dilution of 1:1000 to 1:5000 is recommended. A dilution of 1:500 is recommended for ECL WB on mammalian fibrillarin. A dilution of 1:1000 to 1:5000 is recommended for ICC on yeast cells and 1:500 for mammalian cells. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.

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Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG
• Clone No.: 38F3
• Immunogen: Yeast nuclear preparations. Hybridomas were screened by immunofluorescence on yeast cells and by western blotting on yeast protein homogenates (S. cerevisiae).
• Target: rRNA 2'O-methyltransferase fibrillarin (Nop1p)
• Molecular Weight: 34 kDa
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Lyophilized hybridoma cell culture media with sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

rRNA 2'-O-methyltransferase fibrillarin; 34 kDa nucleolar scleroderma antigen; FBL; FIB1; FLRN; Nop1p

Quick Database Links

Gene Symbol

rRNA 2'O-methyltransferase fibrillarin (Nop1p)

UniProt

P15646