Fibrillarin Antibody

SKU: orb319518

Description

Our Anti-Fibrillarin primary antibody from Biorbyt is mouse monoclonal. It detects human, mouse, and rat Fibrillarin and is Protein G purified.

Research Area

Cell Biology, Disease Biomarkers

Images & Validation

• Tested Applications: ELISA, ICC, IHC, WB
• Dilution Range: WB: 0.736111111, IHC: 1:100-1:500, ICC: 1:100-1:500
• Reactivity: Bovine, Drosophila, Gallus, Human, Mouse, Porcine, Rat
• Predicted Reactivity: Canine, Equine, Feline, Goat, Guinea pig, Hamster, Primate, Rabbit, Rodent, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: 38F3
• Immunogen: Yeast nuclear preparation
• Target: Fibrillarin
• Molecular Weight: 34
• Purification: Protein G Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS + 50% glycerol and 5 mm NaN3
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

34 kD nucleolar scleroderma antigen antibody, 34 kDa nucleolar scleroderma antigen antibody, Fbl antibody, FBRL_HUMAN antibody, FIB antibody, FIB1 antibody, FLRN antibody, Histone-glutamine methyltransferase antibody, Nop1p antibody, RNA U3 small nucleolar interacting protein 1 antibody, RNU3IP1 antibody, rRNA 2' O methyltransferase fibrillarin antibody, rRNA 2'-O-methyltransferase fibrillarin antibody

High magnification confocal image of HeLa cells showing strong, prominent labeling of the nucleoli in the nucleus with Anti-Fibrillarin (Cat. orb319518, 1:100, green), colabeled with Anti-Vimentin (Cat. orb319707, red, 1:1000) showing the cytoplasmic intermediated filaments, and nuclear staining was done with DAPI (blue).

Immunofluorescence of human neuroblastoma SH-SY5Y cells showing strong, prominent labeling of the nucleoli in the nucleus with Anti-Fibrillarin (Cat. orb319518, 1:500, green), and nuclear staining was done with DAPI (blue) which makes the fibrillarin marker appear pale blue.

Western blot of HeLa cell lysate showing specific immunolabeling of the ~34 kDa fibrillarin protein.

Quick Database Links

Gene Symbol

Fibrillarin

UniProt

P15646