FHL1 Antibody

• Catalog Number: orb1257478
• Category: Antibodies
• Description: FHL1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 27-296 of human FHL1 (NP_001153171.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 40kDa
• Target: FHL1
• UniProt ID: Q13642
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: FHL1, FHL1B, KYO-T, MGC111107, SLIM1, bA535K18.1,
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:100
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using FHL1 antibody (orb1257478) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded human thyroid cancer using FHL1 Antibody (orb1257478) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse kidney using FHL1 Antibody (orb1257478) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of HeLa cells using FHL1 antibody (orb1257478). Blue: DAPI for nuclear staining.