FH Antibody

• Catalog Number: orb627033
• Category: Antibodies
• Description: Rabbit polyclonal antibody to FH
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IF, IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: fumarate hydratase
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:500-1:2000; IP: 1:500-1:2000; IHC: 1:20-1:200; IF: 1:10-1:100
• Form/Appearance: liquid
• Purity: ≥95% as determined by SDS-PAGE
• Conjugation: Unconjugated
• MW: 45-50 kDa
• Target: FH
• UniProt ID: P07954
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescent analysis of HepG2 cells using orb627033 (FH antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).

IP Result of anti-FH (IP: orb627033, 4 ug; Detection: orb627033 1:1000) with HepG2 cells lysate 2000 ug.

HeLa cells were subjected to SDS PAGE followed by western blot with orb627033 (FH antibody) at dilution of 1:1000.

Immunohistochemistry of paraffin-embedded human ovary tumor using orb627033 (FH antibody) at dilution of 1:50.