FGFR2 Antibody (C-term)

• Catalog Number: orb1929045
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB1569
• Tested applications: IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:1000, IHC-P-Leica: 1:500
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 92025 Da
• Target: This FGFR2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 794-821 amino acids from the C-terminal region of human FGFR2.
• UniProt ID: P21802
• NCBI: NP_001138388.1, NP_001138389.1, NP_075259.4, NP_001138391.1, NP_001138386.1, NP_000132.3, NP_001138385.1, NP_001138387.1, NP_001138390.1, NP_075418.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Fibroblast growth factor receptor 2, FGFR-2, K-sam
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue was performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

All lanes: Anti-FGFR2 Antibody (C-term) at 1:1000 dilution. Lane 1: NCI-H460 whole cell lysate. Lane 2: HT-29 whole cell lysate. Lane 3: A549 whole cell lysate. Lane 4: T47D whole cell lysate. Lane 5: Hela whole cell lysate. Lane 6: NIH/3T3 whole cell lysate. Lane 7: Mouse brain lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 92 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.