FGFR1 Antibody (C-term)

• Catalog Number: orb1926703
• Category: Antibodies
• Description: Purified Mouse Monoclonal Antibody (Mab)
• Target: This FGFR1 antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 806-842 amino acids from the C-terminal region of human FGFR1.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG1,k
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• UniProt ID: P11362
• MW: 91868 Da
• Tested applications: IF, IHC-P, WB
• Dilution range: IF: 1:25, WB: 1:2000, IHC-P: 1:25
• Clone Number: 1440CT772.50.23
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Fibroblast growth factor receptor 1, FGFR-1, Basic
• Note: For research use only

Staining FGFR1 in human brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Western blot analysis of lysate from Hela cell line, using FGFR1 Antibody (C-term). Diluted at 1:2000. A goat anti-mouse IgG H&L (HRP) at 1: 3000 dilution was used as the secondary antibody. Lysate at 20 μg.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling FGFR1 at 1/25 dilution, followed by Dylight 488-conjugated goat anti-mouse IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on HeLa cell line. The nuclear counter stain is DAPI (blue).