Fgf14 Antibody

• Catalog Number: orb1248557
• Category: Antibodies
• Description: Fgf14 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Bovine, Canine, Porcine, Rat
• Reactivity: Human, Mouse
• Immunogen: The immunogen for this antibody is: REQHWDRPSASR-C
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 28kDa band observed in Mouse and Human Brain lysates (calculated MW of 28.3kDa according to NP_997550.1 and 27.7kDa according to NP_034331.2). Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Cryosection of immersion-fixed (1% PFA/0.5% methanol) Human Hypothalamus. Optimal dilution: 1:5000. *Vibratome prepared sample for optimal labelling.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Approx 28kDa
• Target: Fgf14
• UniProt ID: Q92915
• NCBI: NP_997550.1, NP_034331.2
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: Fgf14, fibroblast growth factor 14, FHF-4, Fhf4, m
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 28kDa band observed in Mouse and Human Brain lysates (calculated MW of 28.3kDa according to NP_997550.1 and 27.7kDa according to NP_034331.2). Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Cryosection of immersion-fixed (1% PFA/0.5% methanol) Human Hypothalamus. Optimal dilution: 1:5000. *Vibratome prepared sample for optimal labelling.
• Expiration Date: 12 months from date of receipt.

orb1248557 (1 ug/ml) staining of Mouse Brain lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1248557 (1:5000) immunostaining of FGF14-positive axon initial segments in cryosection of an immersion-fixed (1% PFA/0.5% methanol) human hypothalamus*. Antigen retrieval with citrate buffer pH 6 at 80C for 30min.