Fgf14 (mouse N terminus) Antibody

• Catalog Number: orb12272
• Category: Antibodies
• Description: Goat polyclonal antibody to Fgf14
• Target: Fgf14 (mouse N terminus)
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Canine, Porcine, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: REQHWDRPSASR
• MW: 27.7; 28.3
• Tested applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:128000, WB: 1-3 μg/ml, IHC-P: 1:5000
• Application notes: WB: Approx 28kDa band observed in Mouse and Human Brain lysates (calculated MW of 28.3kDa according to NP_997550.1 and 27.7kDa according to NP_034331.2). Recommended concentration: 1-3µg/ml. Primary incubation was 1 hour.. IHC: Cryosection of immersion-fixed (1% PFA/0.5% methanol) Human Hypothalamus. Optimal dilution: 1:5000. *Vibratome prepared sample for optimal labelling.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti Fgf14 antibody, anti FGF-14 antibody, anti Fh
• Note: For research use only
• Entrez: 2259

Western blot analysis of Mouse Brain lysate using Fgf14 antibody