FERULIC ACID METHYL ESTER

SKU: orb1297663

Description

Methyl ferulate is a lipophilic, naturally occurring phenolic compound derived from plants like giant fennel. Its enhanced cell permeability makes it a valuable research tool for studying antioxidant, anti-inflammatory, and potential chemopreventive mechanisms in both in vitro and in vivo models.

Research Area

Cell Biology, Signal Transduction

Key Properties

• CAS Number: 2309-07-1
• MW: 208.21
• Purity: 99.91%
• Formula: C₁₁H₁₂O₄
• SMILES: COC(=O)\C=C\c1ccc(O)c(OC)c1
• Target: Autophagy,p38 MAPK
• Solubility: DMSO:50 mg/mL (240.14 mM);Ethanol:20 mg/mL (96.06 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (9.61 mM)

Bioactivity

• In Vitro:
  Ferulic acid methyl ester has less antioxidant capacity than ferulic acid in neuronal PC12 cells (IC50 = 74.7 μM for ferulic acid ethyl ester vs. 44.6 μM for ferulic acid, 2,2-diphenyl-1-picrylhydrazyl radical scavenging). Ferulic acid methyl ester, at 10-25 μg/ml, inhibits the release of pro-inflammatory cytokines, blocks the expression of COX-2, and reduces nitric oxide generation from LPS-stimulated macrophages.
• Cell Research:
  Hydrogen peroxide (8.8 M solution) stored at 4 C was firstdiluted in PBS to prepare a 100 mM solution on the day of theexperiment. This was further diluted in growth medium to preparethe final working solution. PC12 cells were plated in collagencoated96-well microplates at a density of 5 105 cells/ml(100 μl per well). Blank wells contained only growth medium forbackground correction. After 48 h of incubation to allow for cellattachment, 20 μl of growth medium supplemented with differentconcentrations of HCAs were added in triplicate wells and preincubatedfor 1 h. Maximum concentration of DMSO in the wellswas kept below 0.2%. Afterwards, 20 μl of H2O2 solution was added.The concentration of H2O2 in the well was 75 μM. After anotherhour, the medium was replaced with fresh one and cells wereincubated overnight. In the end, the medium was replaced with30 μl of MTT 0.5 mg/ml dissolved in RPMI without phenol red.Formazan crystals were solubilised in 200 μl DMSO after 1.5 h ofincubation at 37 C.

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

p38MAPK, p38 MAPK, Autophagy, Inhibitor, inhibit, Methyl 4'-hydroxy-3'-methoxycinnamate, Methyl ferulate, FERULIC ACID METHYL ESTER

Compound Identifiers

PubChem CID

5357283