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FBXW7 Antibody

Catalog Number: orb1257280

DispatchUsually dispatched within 5-10 working days
$ 800.00
Catalog Numberorb1257280
CategoryAntibodies
DescriptionFBXW7 Antibody
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, IHC, WB
ReactivityHuman, Mouse, Rat
IsotypeIgG
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 408-707 of human FBXW7 (NP_361014.1).
Antibody TypePrimary Antibody
Concentrationbatch dependent
Form/AppearanceLiquid
ConjugationUnconjugated
MWObserved: 80kDa
TargetFBXW7
UniProt IDQ969H0
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesLiquid
Alternative namesFBXW7, AGO, CDC4, DKFZp686F23254, FBW7, FBX30, FBX
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NoteFor research use only
Application notesWB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
Expiration Date12 months from date of receipt.
FBXW7 Antibody

Western blot analysis of extracts of various cell lines, using FBXW7 antibody (orb1257280) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

FBXW7 Antibody

Immunohistochemistry of paraffin-embedded mouse lung using FBXW7 antibody (orb1257280) at dilution of 1:100 (40x lens).

FBXW7 Antibody

Immunohistochemistry of paraffin-embedded mouse kidney using FBXW7 antibody (orb1257280) at dilution of 1:100 (40x lens).

FBXW7 Antibody

Immunofluorescence analysis of U2OS cells using FBXW7 antibody (orb1257280) at dilution of 1:100. Blue: DAPI for nuclear staining.

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