FBXW7 Rabbit Polyclonal Antibody

• Catalog Number: orb580048
• Category: Antibodies
• Description: Rabbit polyclonal antibody to FBXW7
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Predicted Reactivity: Bovine, Canine, Equine, Guinea pig, Mouse, Rabbit, Rat, Zebrafish
• Reactivity: Human
• Immunogen: The immunogen is a synthetic peptide directed towards the C-terminal region of Human FBXW7
• Concentration: 0.5 mg/ml
• Form/Appearance: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Conjugation: Unconjugated
• MW: 80 kDa
• Target: FBXW7
• UniProt ID: Q969H0
• Protein Sequence: Synthetic peptide located within the following region: LKTGEFIRNLVTLESGGSGGVVWRIRASNTKLVCAVGSRNGTEETKLLVL
• NCBI: NP_001013433
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Alternative names: AGO, CDC4, FBW6, FBW7, hAgo, FBX30, FBXW6, SEL10,
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 3 ug/ml of the antibody was used in this experiment. Isoforms present at 70 kDa, 66 kDa, and 59 kDa. Protein is also phosphorylated.

Sample Tissue: Human Jurkat Whole Cell, Antibody dilution: 1 ug/ml.

Sample Tissue: Human Jurkat Whole Cell, Antibody dilution: 1 ug/ml.

Sample Tissue: Human Jurkat Whole Cell, Antibody dilution: 1 ug/ml.

Sample Type: 721_B Whole Cell lysates, Antibody dilution: 1.0 ug/ml.

Sample Type: 721_B Whole Cell lysates, Antibody dilution: 1.0 ug/ml.

Sample Type: 721_B Whole Cell lysates, Antibody dilution: 1 ug/ml.

Sample Type: Human Fetal Heart, Antibody dilution: 1.0 ug/ml.

Kidney

Rabbit Anti-FBXW7 Antibody, Catalog Number: orb580048, Formalin Fixed Paraffin Embedded Tissue: Human Pineal Tissue, Observed Staining: Cytoplasmic in cell bodies and processes of pinealocytes, Primary Antibody Concentration: 1:100, Other Working Concentrations: 1/600, Secondary Antibody: Donkey anti-Rabbit-Cy3, Secondary Antibody Concentration: 1:200, Magnification: 20X, Exposure Time: 0.5-2.0 sec.

WB Suggested Anti-FBXW7 Antibody Titration: 0.2-1 ug/ml, Positive Control: Jurkat cell lysate. FBXW7 is supported by BioGPS gene expression data to be expressed in Jurkat.