FBXW2 Antibody

• Catalog Number: orb1247518
• Category: Antibodies
• Description: FBXW2 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Bovine, Canine, Porcine
• Reactivity: Human, Mouse, Rat
• Immunogen: The immunogen for this antibody is: C-KRGSSFLAGEHPG
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 48kDa band observed in Human Kidney lysates (calculated MW of 48.0kDa according to NP_036296.1). Recommended concentration: 0.3-1.5ug/ml. A stronger band of unknown identity was also consistently observed at appr. 30kDa - both bands were successfully blocked by incubation with the immunising peptide.Immunohistochemistry:In paraffin embedded Human Kidney, shows cytoplasmic staining of epithelial cells in some renal tubules. Recommended concentration, 2-3ug/ml. An anonymous customer found positive results in IHC on Mouse kidney tissue. An anonymous customer found positive results in IHC on Rat kidney tissue.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: FBXW2
• UniProt ID: Q9UKT8
• NCBI: NP_036296.2
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: FBXW2, F-box and WD-40 domain protein 2, FBW2, Fwd
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 48kDa band observed in Human Kidney lysates (calculated MW of 48.0kDa according to NP_036296.1). Recommended concentration: 0.3-1.5ug/ml. A stronger band of unknown identity was also consistently observed at appr. 30kDa - both bands were successfully blocked by incubation with the immunising peptide.Immunohistochemistry:In paraffin embedded Human Kidney, shows cytoplasmic staining of epithelial cells in some renal tubules. Recommended concentration, 2-3ug/ml. An anonymous customer found positive results in IHC on Mouse kidney tissue. An anonymous customer found positive results in IHC on Rat kidney tissue.
• Expiration Date: 12 months from date of receipt.

orb1247518 staining (0.3 ug/ml) of Human Kidney lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

orb1247518 (3 ug/ml) staining of paraffin embedded Human Kidney. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.