FASN Rabbit Polyclonal Antibody

• Catalog Number: orb10644
• Category: Antibodies
• Description: FASN Rabbit Polyclonal Antibody
• Target: FASN
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Mouse, Sheep
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide derived from humanFASN (1451-1550/2411aa)
• UniProt ID: P49327
• RRID: AB_10752626
• MW: 272 kDa
• Tested applications: ELISA, IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: fatty acid synthase; FASN; FAS; FA Synthase 1; MGC
• Research Area: Cancer Research, Cell Biology, Energy Metabolism,
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Sample: Lane 1: Human HeLa cell lysates, Lane 2: Human HepG2 cell lysates, Primary: Anti-FASN (orb10644) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 272 kDa, Observed band size: 250 kDa.

Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-FASN Polyclonal Antibody, Unconjugated (orb10644) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.