FASN Rabbit Polyclonal Antibody

SKU: orb10644

Description

FASN Rabbit Polyclonal Antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: ELISA, IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
• Reactivity: Human
• Predicted Reactivity: Mouse, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from humanFASN (1451-1550/2411aa)
• Target: FASN
• Molecular Weight: 250 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

FAS; OA-519; SDR27X1; A630082H08Rik; FAS_CHICK; FASN; 2.3.1.85; FAS_BOVIN; FAS_HUMAN; Type I fatty acid synthase; FAS_MOUSE; FAS_RAT; Type I FAS;

Sample: Lane 1: Human HeLa cell lysates, Lane 2: Human HepG2 cell lysates, Primary: Anti-FASN (orb10644) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 272 kDa, Observed band size: 250 kDa.

Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-FASN Polyclonal Antibody, Unconjugated (orb10644) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

FASN

UniProt

P49327