FASN Mouse Monoclonal Antibody

SKU: orb500057

Description

FASN Mouse Monoclonal Antibody

Images & Validation

• Tested Applications: ICC, IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-5000, IHC-P=1:100-500, IHC-F=1:20-200, ICC/IF=1:100-500, IF=1:20-200
• Reactivity: Human
• Predicted Reactivity: Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1, k
• Clone No.: 2C5
• Immunogen: KLH conjugated synthetic peptide derived from human FASN
• Target: FASN
• Molecular Weight: 274 kDa
• Purification: Affinity purified by Protein AGL
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

fatty acid synthase; FASN; FAS; FA Synthase 1; MGC14367; MGC15706; OA-519; Fatty acid synthase; [Acyl-carrier-protein] S-acetyltransferase; [Acyl-carrier-protein] S-malonyltransferase; 3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase; 3-oxoacyl-[acyl-carrier-protein] reductase; 3-oxoacyl-[acyl-carrier-protein] synthase; Enoyl-[acyl-carrier-protein] reductase; Oleoyl-[acyl-carrier-protein] hydrolase; FAS_MHUMAN.

Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (FASN) Monoclonal Antibody, Unconjugated (orb500057) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human breast), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (FASN) Monoclonal Antibody, Unconjugated (orb500057) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (FASN) Monoclonal Antibody, Unconjugated (orb500057) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (FASN) Monoclonal Antibody, Unconjugated (orb500057) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Sample: Lane 1: Human HeLa cell lysates, Lane 2: Human HepG2 cell lysates, Primary: Anti-FASN (orb500057) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 274 kDa, Observed band size: 270 kDa.

Quick Database Links

Gene Symbol

FASN

UniProt

P49327